| Theme | The Front of Dialysate Purification Technologies in Hemodialysis Therapy | |
|---|---|---|
| Title | Microbiological monitoring of hemodialysis fluids | |
| Publish Date | 2007/05 | |
| Author | Yuzo Kan-no | Department of Blood Purification, Tokyo Medical and Dental University |
| [ Summary ] | Microbiological surveillance of hemodialysis fluids is very important in relation to strategies for bacterial contamination of dialysate distribution systems. The common microbiological culturing method uses petridishes with a layer of agar. A spread plate count is one basic method to produce microbiological cultures. Moreover, Resoner's No. 2 Agar (R2A) and Tryptone Glucose Extract Agar (TGEA) are the recommended mediums for heterotrophic bacteria cultures in dialysates. A spread plate count technique is to sample 1 ml spread over the surface of a petridish with an agar medium. After 7 days of incubation at between 15-25 °C the numbers of colonies were quantified. Anyone can easily perform spread plate counts. They are the first choice for investigation of hemodialysis fluids. |
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