| Theme | New Trends in Peritoneal Dialysis Technologies | |
|---|---|---|
| Title | Translocation of aquaporin-1 to plasma membrane induced by hyperosmotic stimuli effect on ultrafiltration in peritoneal dialysis | |
| Publish Date | 2003/05 | |
| Author | Shingo Kuboshima | St.Marianna University School of Medicine, Department of Internal Medicine, Division of Nephrology and Hypertension |
| Author | Tsutomu Sakurada | St.Marianna University School of Medicine, Department of Internal Medicine, Division of Nephrology and Hypertension |
| Author | Goichi Ogimoto | St.Marianna University School of Medicine, Department of Internal Medicine, Division of Nephrology and Hypertension |
| [ Summary ] | Aquaporin-1 (AQP 1) has been reported to play an important role in ultrafiltration through the peritoneum in peritoneal dialysis. To determine the precise mechanism involved in this process, the present study was designed to examine the translocation of AQP 1 to the plasma membrane, induced by hyperosmotic stimuli. Cultured RPMC obtained were incubated in a medium with or without glucose or mannitol as the hyperosmotic stimuli, at room temperature. Significant enhancement of AQP 1 quantity by the addition of 5% glucose to medium was observed at 2.5 minutes, followed by a sustained increase in quantity over the next fifteen minutes. AQP 1 quantities were also significantly increased by the addition of 5% mannitol. These results suggest that hyperosmotic stimuli could generate an augmentation of AQP 1 quantity in the plasma membrane by the translocation of AQP 1. This process, in the short term, might contribute to the mechanism involved in ultrafiltration through the peritoneum in peritoneal dialysis. |
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