| Theme | Homocysteinemia in Association with Chronic Renal Failure | |
|---|---|---|
| Title | Analytical methods for measurement of plasma total homocysteine concentrations | |
| Publish Date | 1999/02 | |
| Author | Hiroaki Ono | Department of Pediatrics, Hiroshima University School of Medicine |
| Author | Akiko Sakamoto | Department of Pediatrics, Hiroshima University School of Medicine |
| Author | Nobuo Sakura | Department of Pediatrics, Hiroshima University School of Medicine |
| [ Summary ] | Today the level of plasma total homocysteine (free homocysteine and protein-bound homocysteine;tHcy) is mostly used as a marker of folate and cobalamin functions and in cardiovascular risk assessment. Values for tHcy have been obtained by different methods, such as radioenzymatic assay, gas chromatography-masss pectrometry, high performance liquid chromatography (HPLC) and immunoassay. We have measured plasma tHcy concentrations of 130 normal control subjects and 2 homocystinuric patients, using HPLC of fluorescent compounds obtained after precolumn derivation. This method is clinically useful, since the sample volume is very small and it is quick and simple. In this section, methods for plasma tHcy determination, including ours, have been reviewed and the values of plasma tHcy concentrations of control subjects and patients has been shown. | |