| Theme | The front line of magnifying endoscopy and pit pattern diagnosis -- Now and future | |
|---|---|---|
| Title | Real time virtual histology in vivo of colorectal lesions using Endo-Cytoscopy system | |
| Publish Date | 2005/03 | |
| Author | Keita Sasajima | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Shin-ei Kudo | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Tsukasa Takeuchi | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Hiroshi Kashida | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Haruhiro Inoue | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Eiji Hidaka | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Hiroshi Kawauchi | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Masanori Sakashita | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Yoshio Omae | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Yui Kudo | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Nobuya Satake | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Yasuhiro Omori | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Junichi Tanaka | Digestive Disease Center, Showa University Northern Yokohama Hospital |
| Author | Akira Shiokawa | Division of Pathology, Showa University Northern Yokohama Hospital |
| [ Summary ] | Background : The histological findings of gastrointestinal lesions are on the basis of light-microscopic examination of hematoxylin-eosin stained thin-slice specimens. Endo-Cytoscopy (E-C) system, novel ´ultra-high´ magnifying endoscopy enables cellular level of microscopic observation in vivo and can be applied clinically. A study of the observation of colorectal lesions using E-C to obtain real-time histological images in vivo during endoscopy was performed. Methods : Seventy-five colorectal lesions were examined using E-C. After spraying 1 % methylene blue dye, the instrument was passed through the working channel of the colonoscope. In order to obtain real time images of the lesions at cellular level in vivo, the tip of the instrument needs to be touched to the target. Diagnosis of E-C images was done by a pathologist who was blinded to the histological diagnosis. All lesions were resected endoscopically or surgically after E-C observation were made. The E-C images for each lesion were compared with hematoxylin-eosin stained horizontal cross-sections. Results : It was possible to distinguish neoplastic lesions from non-neoplastic, and also possible to distinguish invasive cancer from adenomas. Conclusions : ´Ultra-high´ magnifying endoscopy, E-C system, provides real-time histological images in vivo that correspond well with those of hematoxylin-eosin stained microscopic images. |
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