| Theme |
Cell biology of tooth |
| Title |
Molecular and functional characterization of dental pulp cells |
| Author |
Yuko Nakamichi |
Institute for Oral Science, Matsumoto Dental University |
| Author |
Naoyuki Takahashi |
Institute for Oral Science, Matsumoto Dental University |
| Author |
Nobuyuki Udagawa |
Department of Biochemistry, Matsumoto Dental University |
| [ Summary ] |
Dental pulp is usually maintained in a non-mineralized state despite being located inside a highly mineralized tooth. The extracellular matrix (ECM) of pulpal fibroblasts obtained from human and mouse teeth are easily mineralized when cultured under certain conditios. This ability of pulpal fibroblast ECM is much higher than that of osteoblasts and bone marrow stromal cells. Moreover, the gene expression profiles of cultured pulpal fibroblasts are similar to those of the other two cell types. Pulpal fibroblasts also promote osteoclastogenesis when cocultured with monocytic cells. Furthermore, the transplantation of three-dimensional cultures of human and mouse pulpal fibroblasts onto muscles of immunocompromised mice can effectively regenerate bones with marrow cavities. Based on our results, obtained using molecular and cellular biological approaches, we discuss the significance of pulpal fibroblasts as biomaterial for hard tissue regeneration. |