| 特集名 | 骨・軟骨の再生 | |
|---|---|---|
| 題名 | 滑膜間葉系幹細胞由来スキャフォールドフリー三次元人工組織・人工骨複合体による骨軟骨再生 | |
| 発刊年月 | 2015年 10月 | |
| 著者 | 下村 和範 | 大阪大学大学院医学系研究科健康スポーツ科学講座スポーツ医学 |
| 著者 | 藤江 裕道 | 首都大学東京システムデザイン学部 |
| 著者 | 吉川 秀樹 | 大阪大学大学院医学系研究科整形外科 |
| 著者 | 中村 憲正 | 大阪保健医療大学スポーツ医科学研究所／大阪大学臨床医工学融合研究教育センター |
| 【 要旨 】 | 骨軟骨病変に対する生物学的再建のためには，軟骨および軟骨下骨の層別の再生が望ましい．われわれは滑膜間葉系幹細胞由来スキャフォールドフリー三次元人工組織（TEC）および連通多孔体ハイドロキシアパタイト人工骨より，TEC・人工骨複合体を開発し，骨軟骨再生への有用性を検討した． 家兎の右大腿骨顆間部に骨軟骨欠損を作製し，TEC・人工骨複合体を移植した（T群）．コントロール群は人工骨のみ移植した（C群）．術後，組織学的評価および生体力学的評価を行った． 術後6カ月で，C群では一定の骨軟骨形成が認められたが，周囲組織との間に亀裂を認め，修復組織の劣化がみられた．一方，T群では，再生骨軟骨は良好に維持され，組織スコアーは有意に高く，また正常軟骨と同等の力学特性が得られた． TEC・人工骨複合体は術後長期においても優れた骨軟骨再生能を呈し，またその安全性も併せ，臨床応用が期待される． |
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| Theme | Regeneration of bone and cartilage | |
|---|---|---|
| Title | Osteochondral repair using a combined implant comprising a scaffold-free tissue-engineered construct derived from synovial mesenchymal stem cells and a hydroxyapatite-based artificial bone | |
| Author | Kazunori Shimomura | Medicine for Sports and Performing Arts, Department of Health and Sport Sciences, Osaka University Graduate School of Medicine |
| Author | Hiromichi Fujie | Faculty of System Design, Tokyo Metropolitan University |
| Author | Hideki Yoshikawa | Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine |
| Author | Norimasa Nakamura | Institute for Medical Science in Sports, Osaka Health Science University / Center for Advanced Medical Engineering and Informatics, Osaka University |
| [ Summary ] | For an ideal osteochondral repair, it is important to facilitate zonal restoration of the subchondral bone and the cartilage, layer by layer. The purpose of the present study was to investigate the feasibility of a combined material comprising a scaffold-free tissue-engineered construct (TEC) derived from synovial MSCs and an HA-based artificial bone using a rabbit osteochondral defect model. Osteochondral defects were created on the femoral groove of skeletally mature rabbits, and the TEC-HA combined implant was then implanted into defects. In the control group, HA alone was implanted. Histological evaluation and biomechanical testing were performed to assess the degree of tissue repair. Osteochondral defects treated with the TEC-HA implants exhibited complete osteochondral repair at 6 months postimplantation and good tissue integration of the repair tissue to the adjacent host tissue. Conversely, the control group exhibited poor integration into adjacent cartilage and early osteoarthritis-like degenerative changes at 6 months postimplantation. Biomechanically, tissue stiffness was resotred in the osteochondral tissue treated with the TEC-HA implants at 6 months postimplantation and was similar to normal osteochondral tissue. The TEC-HA implant represents a promising MSC-based bio-implant with regard to safety and long-term durability. |
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