| 特集名 | 透析液清浄化技術の最前線 | |
|---|---|---|
| 題名 | 細菌検出法の実際 (1) 各種細菌測定法とその注意点 | |
| 発刊年月 | 2007年 05月 | |
| 著者 | 菅野 有造 | 東京医科歯科大学附属病院血液浄化療法部・臨床工学技士 |
| 【 要旨 】 | 透析液の微生物汚染管理は重要であり，その監視には平板塗沫法などの細菌培養法が用いられる．現在，透析液の培養に用いる培地は従属栄養細菌 (グラム陰性菌) の検出に適したReasoner's No.2 Agar (R2A) やTryptone Glucose Extract Agar (TGEA) 培地の使用が推奨されている．平板塗沫法の手技は，検体1 mlをシャーレに注入し，培養温度15 - 25 °Cで7日間保存する．その後，培養により形成されるコロニーを目視にて計数する．平板塗沫法は手技が簡便で，細菌検出を行う第一選択として有用である． | |
| Theme | The Front of Dialysate Purification Technologies in Hemodialysis Therapy | |
|---|---|---|
| Title | Microbiological monitoring of hemodialysis fluids | |
| Author | Yuzo Kan-no | Department of Blood Purification, Tokyo Medical and Dental University |
| [ Summary ] | Microbiological surveillance of hemodialysis fluids is very important in relation to strategies for bacterial contamination of dialysate distribution systems. The common microbiological culturing method uses petridishes with a layer of agar. A spread plate count is one basic method to produce microbiological cultures. Moreover, Resoner's No. 2 Agar (R2A) and Tryptone Glucose Extract Agar (TGEA) are the recommended mediums for heterotrophic bacteria cultures in dialysates. A spread plate count technique is to sample 1 ml spread over the surface of a petridish with an agar medium. After 7 days of incubation at between 15-25 °C the numbers of colonies were quantified. Anyone can easily perform spread plate counts. They are the first choice for investigation of hemodialysis fluids. |
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