| Theme |
Cytokines and Dialysis Treatment |
| Title |
Therapeutic angiogenesis using peripheral blood stem cells for hemodialysis patients |
| Author |
Motoki Yonekawa |
Department of Surgery, Sapporo Hokuyu Hospital |
| Author |
Takashi Horie |
Department of Surgery, Sapporo Hokuyu Hospital |
| Author |
Ichiro Tsuda |
Department of Surgery, Sapporo Hokuyu Hospital |
| Author |
Akio Kawamura |
Department of Surgery, Sapporo Hokuyu Hospital |
| [ Summary ] |
Therapeutic angiogenesis using bone marrow cell transplantation was reported and this therapy was found to be useful for arterio sclerosis obliterans (ASO) treatment. We attempted the same procedure using peripheral blood stem cell collection (PBSCC). One hundred and nineteen patients were included in this study, 84 were hemodialysis patients. They all had peripheral arterial disease (PAD), 109 in the legs and 10 in the arms. Patients were given 5 μg / kg / day G-CSF for four days, subcutaneously. PBSCC was performed on day four. For all patients, 8.3 l peripheral blood was treated with an apheresis machine (CS-3000 or Spectra) and 3.6 x 107 CD 34 cells were harvested, along with mononuclear cells, red blood cells and platelets. The CD 34 cells were not purified with the magnetic bead method. CD 34 enriched fluid (56.9 ml) was injected at 114 points in the arm and leg muscle on the same day. Thirty six patients recovered. Elimination of foot ulcers continued over a four years period. The symptoms of the 29 patients did not change but their extremities were maintained. Fifty four patients required amputations because their ulcers became necrotic or infected. |