| Theme |
Dialysis in Diabetic Patients |
| Title |
Therapeutic angiogenesis for diabetic patients using peripheral blood stem cell collection (PBSCC) |
| Author |
Takashi Horie |
Department of Surgery, Sapporo Hokuyu Hospital |
| Author |
Akio Kawamura |
Department of Surgery, Sapporo Hokuyu Hospital |
| Author |
Ichiro Tsuda |
Department of Surgery, Sapporo Hokuyu Hospital |
| [ Summary ] |
Therapeutic angiogenesis, using bone marrow cell transplantation, has been reported on. This therapy was useful for arterio sclerosis obliterans (ASO). We attempted the same procedure using PBSCC. Sixty-five patients were included in this study, 16 were diabetic hemodialysis patients with gangrene of the limbs. The patients were given 5µg / kg / day G-CSF for 4 days subcutaneously. PBSCC was done on day 4. For each patient, 8 to 10l of peripheral blood was treated with an apheresis machine (CS-3000 or Spectra) and 0.2 to 15.9 × 107 CD34 cells were harvested with mononuclear cells, red blood cells and platelets. We did not purify the CD34 cells using the magnetic beads method. CD34 enriched fluid (35 to 93ml) was injected at 60 to 190 points into limb muscle on the same day. Forty of 65 patients recovered. However, 12 out of 16 gangrenous patients received amputations because their necrotic ulcers were painful and infected. |