| Theme |
Biocompatibility on Materials for Dialysis Equipments and Devices |
| Title |
Possible mimics of osteoblast and osteoclast function associated with the reactive oxygen and extracellular superoxide dismutase in vitro |
| Author |
Harutaka Yamada |
Division of Nephrology and Rheumatology, Aichi Medical University School of Medicine |
| Author |
Yasukazu Yamada |
Division of Nephrology and Rheumatology, Aichi Medical University School of Medicine |
| Author |
Tetsuo Adachi |
Division of Nephrology and Rheumatology, Aichi Medical University School of Medicine |
| Author |
Hirokazu Imai |
Division of Nephrology and Rheumatology, Aichi Medical University School of Medicine |
| [ Summary ] |
In the present study, we have developed an in vitro model to study bone-dynamism in the balance between osteoblasts and osteoclasts. Osteoblasts; hFOB1.19, are cells derived from human osteoblast cell lines and osteoclasts. The human lymphoma cell line U937 was used in this study with differentation conditions. The hFOB1.19 cells expressed the extracellular superoxide dismutase, which is the most important anti-oxidant enzyme in the extracellular space, in relation to differentiation conditions, in accordance with the production of alkali phosphates and cAMP. Differentiated U937 cells expressed the tartrate-resistant acid phosphatase, calcitonin receptors and RANK, which are specific osteoclast markers, and also produce reactive oxygen. These results suggest the osteogenic process, functioning under both of osteoblasts and osteoclasts is comparable in relation to oxidative stress and anti-oxidant enzymes. These systems would also provide a useful tool to analyze the relationship between the long term hemodialysis complication and the oxygen radical damage. |