| Theme |
Laser Endoscopy in Theory and Practice --Perspective of GI Endoscopy in the 21st Century |
| Title |
Endoscopic Fluorescence Observation |
| Author |
Hirofumi Matsui |
Department of Internal Medicine, Tsukuba University |
| Author |
Akira Nakahara |
Department of Internal Medicine, Tsukuba University |
| [ Summary ] |
We previously reported that the gastric mucosa emit fluorescence of porphyrins at the onset of the gastric lesion induced by hemorrhagic shock.In this study, we have established a fluorescence observation system named a simultaneous fluorescence analyzing apparatus (SEFAS), and investigated that whether the fluorescence observation can help us for sensitive and objective diagnosis for acute gastric mucosal lesions. Inthe gastric mucosa treated with indomethacin,lesions generated and myeloperoxidase activity was increased. The indomethacin administration also increased thiobarbituric acid reactive substances, the index of tissue peroxidation. After the indomethacin treatment, the gastric mucosal fluorescence intensities elevated. HPLC analysis demonstrated that the fluorescent substances were mesoporphyrin and protoporphyrin which were the same as found in hemorrhagic shock. Pretreatment of the tissue with radical scavenging substances, catalase and troxipide restrained the increase of mucosal fluorescence intensity and tissue peroxidation. Fluorescence observation with SEFAS can help us for diagnosing tissue peroxidation formation sensitively and objectively. These findings indicate that NSAIDs treatment induced generation of porphyrins as well as tissue peroxidation in gastric mucosal tissue. Fluorescence observation with SEFAS is suggested to provide an objective detection for NSAIDs-induced gastric injury. |