| [ Summary ] |
The goal of this study was to clarify morphogenetic development in relation to isolated crypts and pit patterns in colorectal neoplasms. The HCl-digestion method was used for crypt isolation.
In colorectal tumors with type IIIL pit patterns, isolated crypts of the protruded type displayed greater roughness with budding on their surface. Non-granular type laterally spreading tumors (LST-NG) with type IIIL pit patterns, isolated crypts displayed frontal formation, that is, the surface area was tumorous but bottom was non-tumor. Crypt orifices exhibiting type IIIS pit patterns revealed crypts with or without fission from the bottom. Colorectal neoplasms with depression enlarge throughthe process of crypt bifurcation in which a single crypt divides into two crypts. These results indicate that colorectal neoplasms with a depression enlarge through the process of "crypt fission" in which a single crypt divides into two crypts, LST-NG type neoplasms enlarge through the processof "top-down morphogenesis", and neoplasms with protrusion enlarge by the process of "budding". |