| Theme |
Kampo Medicine : New Trends in GI Therapoitics |
| Title |
Pentagalloylglucose, an Inhibitor of H+,K+-ATPase Purified from Hog Stomach |
| Author |
Kouichi Ono |
The Third Dept. of Internal Medicine, Nihon University, School of Medicine |
| Author |
Ariyoshi Iwasaki |
The Third Dept. of Internal Medicine, Nihon University, School of Medicine |
| [ Summary ] |
We purified a compound with a strong inhibitory effect on H+, K+-ATPase from Paeoniae Radix, which has been used in Japan for the treatment of gastritis and peptic ulcers. The compound was identified as 1,2,3,4,6,-Penta-O-galloyl-bete-D-glucose by proton nuclear magnetic resonance, carbon-13 nuclear magnetic resonance, and fast atomic bombardment mass spectrometry. Pentagalloylglucose also inhibited the accumulation of [14C] aminopyrine in parietal cells isolated from the guinea pig stomach and then stimulated with 10microM histamine (IC50 7.8 /microM) and 1 mM dbc-AMP (IC50 10microM). These results suggest that pentagalloylglucose is a potent inhibitor of H+, K+-ATPase and may be responsible for the inhibition of acid secretion by Paeoniae Radix. |